Products and
Services
Phenotype
Screening Corporation offers products and services to cover a wide
range of applications.
Proposal Work
We can provide scoping studies to support your future proposals.
Depending on the subject of the proposal and other circumstances, we can perform an initial
proof of concept experiment, or we may have already performed similar work that can be used
to demonstrate the feasibility of your proposal. Our role in your proposal can be as a
co-investigator, subcontractor, or as supplier of goods and services. We can assist you by
providing proposal content, illustrations, our budget, CVs, C&P, etc. We have written
successful proposals that have collaborations with academic and commercial entities. The
choice of lead investigator is usually determined by the requirements of the funding source
and the objectives of the proposal.
In House Research
for Customers
Germination and
Growth Service
Phenotype
Screening Corporation will receive your seed samples, germinate them
and grow them in our Plant Growth Container and Substrate sets. These
sets of low density polymeric foam are disease and pest free. All
nutrients, water, stressor materials, disease, pests and temperature are under the
control of the experimenter.
Root Imaging
Service
Plant
root systems grown in our x-ray transparent Plant Growth Container and
Substrate sets can be imaged using one of our low energy digital x-ray
systems. Below figure shows an exploded view of plant, growth
substrate, container, and x-ray imaging geometry.
Rice
plant placed in measurement chamber of our commercial RootViz system. X-Ray
shutter is on left and camera is on right. When x-ray shutter is closed
all x-ray radiation is safely contained. Cabinet door interlocks
prevent accidental exposure to x-rays. Note that plant is rotated 90 degrees prior to imaging.
We maintain an in-house x-ray system for the x-ray
imaging of plant root systems. We use this system to image plants
that we grow in house as well as root systems sent to us by customers. This
system is highly reconfigurable.
Assemble
X-Ray Images into a Photomontage
Depending on
the size of container used and the choice of 2D or stereo imaging, a plant root
system may require anywhere from four to 160 individual images to provide
complete coverage. We have developed software that automatically performs gray
scale enhancement, distortion correction, cropping, appending individual images
into a single overall image of the root system, combination of two images into a
stereo pair and inclusion of textual information.
Analysis of Images
We have developed
segmentation software that can isolate roots from the noisy background of moisture
inclusions in the substrate, shadowing of small roots by larger roots, and varying
gray scale levels of images. We can then apply additional analysis methods that will
be determined by the customer's interests. Below is a reduced resolution assembled
image of a USG5601T soybean root system at day 46. Anaglyphic glasses are required
to see the stereo effect.
The soybean root system
is segmented out using our automated analysis software.
The segmented image is shown below. Note that this image is created without user
input or operator assistance and is ready for further analysis.
Complete X-Ray
Systems
Plant
Scientists who have a large throughput requirement or some kind of
proprietary application may wish to purchase a complete x-ray system. Because of
the wide variety of plants and container sizes, each application will
dictate the details of cabinet size, stage travel, etc. Our standard commercial
system is shown below.
The
commercial cabinet system features three axes of motion for large
photomontages as well as stereo and multi-angle viewing and has a
100 x 100 mm x-ray camera. The fixture for our 200mm wide x 500mm tall container is shown.
Our
x-ray systems are controlled entirely by software, making possible
automated image acquisition sequences of the entire plant container
area.
Because
of the precision and repeatability of our systems, large photomontages
can be accurately and reliably assembled using our support software.
Plant Growth
Containers and Substrate
Phenotype
Screening Corporation offers a wide range of substrate and container
sizes to suit the needs of various plants and growth periods. Sizes
vary from 50mm wide x 200mm tall up to 200mm wide x 1000mm tall. Larger
sizes are possible. Please note that our standard commercial x-ray system has
a maximum capacity for a 200mm x 500mm sized container.
We offer
a choice of growth substrate materials. Our current recommendation is EPS beads.
Customer Support
Phenotype
Screening Corporation supports our customers with on-site and phone support.
We offer on site operator training, hardware and software support for buyers of our
commercial systems. We also offer container selection, growth protocol and
nutrient solution advice to our customers.
Your success is our success.
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